Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies

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Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies.

Monoclonal antibodies specific to the alpha subunits of the photosynthetic coupling factor 1 (CF(1)) were used as marker molecules in an electron microscopic analysis of the subunit organization of this enzyme. Immune complexes were obtained by incubation of CF(1) with saturating amounts of anti-alpha-subunit IgG, isolated by gel filtration, and visualized by electron microscopy. The maximum nu...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1985

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.82.23.7874